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pmt flag dints11 wt puro  (Addgene inc)


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    Addgene inc pmt flag dints11 wt puro
    KEY RESOURCES TABLE
    Pmt Flag Dints11 Wt Puro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmt+flag+dints11+wt+puro/pMT-puro+(Plasmid+%2317923)/pmc06952639-1085-216-214
    Average 93 stars, based on 33 article reviews
    pmt flag dints11 wt puro - by Bioz Stars, 2026-10
    93/100 stars

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    1) Product Images from "The Integrator complex attenuates promoter-proximal transcription at protein-coding genes"

    Article Title: The Integrator complex attenuates promoter-proximal transcription at protein-coding genes

    Journal: Molecular cell

    doi: 10.1016/j.molcel.2019.10.034

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Recombinant, Plasmid Preparation, Software

    Related Articles

    Recombinant:

    Article Title: The Integrator complex attenuates promoter-proximal transcription at protein-coding genes
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IntS1 Ezzeddine et al., 2011 IntS9 Ezzeddine et al., 2011 IntS11 Ezzeddine et al., 2011 alpha-tubulin abcam ab15246 AB_301787 H3K4me3 EpiCypher 13-0028 Normal Rabbit serum Invitrogen 01-610-1 Chemicals, Peptides, and Recombinant Proteins AzNTPs TriLink Technologies Cat# K-1005 Biotin-11-NTPs Perkin Elmer Cat# NEL54(2/3/4/5)001 Critical Commercial Assays NEBNext Ultra II DNA library kit NEB Cat# E7103S Deposited Data Raw and analyzed data This paper GEO: {"type":"entrez-geo","attrs":{"text":"GSE114467","term_id":"114467"}} GSE114467 Start-seq from Triptolide-treated S2 cells Krebs et al., 2017 GEO: {"type":"entrez-geo","attrs":{"text":"GSE77369","term_id":"77369"}} GSE77369 H3K4me1, H3K4me3 and H3K36me3 ChIP-seq from S2 cells Henriques et al., 2018 GEO: {"type":"entrez-geo","attrs":{"text":"GSE85191","term_id":"85191"}} GSE85191 Start-seq from Control and Rrp40-depleted S2 cells Henriques et al., 2013 GEO: {"type":"entrez-geo","attrs":{"text":"GSE49078","term_id":"49078"}} GSE49078 Chromatin RNA-seq from IntS11-depleted HeLa cells Lai et al., 2015 GEO: {"type":"entrez-geo","attrs":{"text":"GSE68401","term_id":"68401"}} GSE68401 H3K4me1, H3K4me3 and H3K36me3 ChIP-seq from HeLa cells Gerstein et al., 2012 GEO: {"type":"entrez-geo","attrs":{"text":"GSE29611","term_id":"29611"}} GSE29611 PRO-seq from DMSO-treated HeLa cells Nilson et al. 2017 GEO: {"type":"entrez-geo","attrs":{"text":"GSE100742","term_id":"100742"}} GSE100742 Chromatin RNA-seq from Xrn2 or CPS73-depleted HeLa cells Nojima et al., 2015 GEO: {"type":"entrez-geo","attrs":{"text":"GSE60358","term_id":"60358"}} GSE60358 Experimental Models: Cell Lines DL1 cells Dr. Sara Cherry, UPenn S2-DGRC clone 6 DGRC Stock number 6 DL1 FLAG-eGFP This paper DL1 FLAG-IntS11WT This paper DL1 FLAG-IntS11E203Q This paper Oligonucleotides Table S3 This paper Recombinant DNA pUB-3xFLAG vector Chen et al., 2012 pMT-puro David Sabatini lab Addgene #17923 pMT-FLAG-dIntS11-WT-puro This paper pMT-FLAG-dIntS11-E203Q-puro This paper pMT-FLAG-eGFP-puro This paper Software and Algorithms bowtie 1.2.2 Langmead et al., 2009 R v3.3.1 www.r-project.org Rstudio v1.0.136 www.rstudio.com featureCounts Liao et al., 2014 DESeq2 Love et al. , 2014 MISO Katz et al., 2010 Prism v8.1.2 GraphPad Partek Genomics Suite v6.15.0127 www.partek.com Open in a separate window KEY RESOURCES TABLE Integrator inhibits transcription elongation at ~15% of mRNA genes and enhancers Integrator targets promoter-proximally paused Pol II for termination The RNA endonuclease of Integrator subunit 11 is critical for gene repression Integrator-depletion increases productive elongation and Histone H3 K4 methylation

    Chromatin Immunoprecipitation:

    Article Title: The Integrator complex attenuates promoter-proximal transcription at protein-coding genes
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IntS1 Ezzeddine et al., 2011 IntS9 Ezzeddine et al., 2011 IntS11 Ezzeddine et al., 2011 alpha-tubulin abcam ab15246 AB_301787 H3K4me3 EpiCypher 13-0028 Normal Rabbit serum Invitrogen 01-610-1 Chemicals, Peptides, and Recombinant Proteins AzNTPs TriLink Technologies Cat# K-1005 Biotin-11-NTPs Perkin Elmer Cat# NEL54(2/3/4/5)001 Critical Commercial Assays NEBNext Ultra II DNA library kit NEB Cat# E7103S Deposited Data Raw and analyzed data This paper GEO: {"type":"entrez-geo","attrs":{"text":"GSE114467","term_id":"114467"}} GSE114467 Start-seq from Triptolide-treated S2 cells Krebs et al., 2017 GEO: {"type":"entrez-geo","attrs":{"text":"GSE77369","term_id":"77369"}} GSE77369 H3K4me1, H3K4me3 and H3K36me3 ChIP-seq from S2 cells Henriques et al., 2018 GEO: {"type":"entrez-geo","attrs":{"text":"GSE85191","term_id":"85191"}} GSE85191 Start-seq from Control and Rrp40-depleted S2 cells Henriques et al., 2013 GEO: {"type":"entrez-geo","attrs":{"text":"GSE49078","term_id":"49078"}} GSE49078 Chromatin RNA-seq from IntS11-depleted HeLa cells Lai et al., 2015 GEO: {"type":"entrez-geo","attrs":{"text":"GSE68401","term_id":"68401"}} GSE68401 H3K4me1, H3K4me3 and H3K36me3 ChIP-seq from HeLa cells Gerstein et al., 2012 GEO: {"type":"entrez-geo","attrs":{"text":"GSE29611","term_id":"29611"}} GSE29611 PRO-seq from DMSO-treated HeLa cells Nilson et al. 2017 GEO: {"type":"entrez-geo","attrs":{"text":"GSE100742","term_id":"100742"}} GSE100742 Chromatin RNA-seq from Xrn2 or CPS73-depleted HeLa cells Nojima et al., 2015 GEO: {"type":"entrez-geo","attrs":{"text":"GSE60358","term_id":"60358"}} GSE60358 Experimental Models: Cell Lines DL1 cells Dr. Sara Cherry, UPenn S2-DGRC clone 6 DGRC Stock number 6 DL1 FLAG-eGFP This paper DL1 FLAG-IntS11WT This paper DL1 FLAG-IntS11E203Q This paper Oligonucleotides Table S3 This paper Recombinant DNA pUB-3xFLAG vector Chen et al., 2012 pMT-puro David Sabatini lab Addgene #17923 pMT-FLAG-dIntS11-WT-puro This paper pMT-FLAG-dIntS11-E203Q-puro This paper pMT-FLAG-eGFP-puro This paper Software and Algorithms bowtie 1.2.2 Langmead et al., 2009 R v3.3.1 www.r-project.org Rstudio v1.0.136 www.rstudio.com featureCounts Liao et al., 2014 DESeq2 Love et al. , 2014 MISO Katz et al., 2010 Prism v8.1.2 GraphPad Partek Genomics Suite v6.15.0127 www.partek.com Open in a separate window KEY RESOURCES TABLE Integrator inhibits transcription elongation at ~15% of mRNA genes and enhancers Integrator targets promoter-proximally paused Pol II for termination The RNA endonuclease of Integrator subunit 11 is critical for gene repression Integrator-depletion increases productive elongation and Histone H3 K4 methylation

    RNA Sequencing Assay:

    Article Title: The Integrator complex attenuates promoter-proximal transcription at protein-coding genes
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IntS1 Ezzeddine et al., 2011 IntS9 Ezzeddine et al., 2011 IntS11 Ezzeddine et al., 2011 alpha-tubulin abcam ab15246 AB_301787 H3K4me3 EpiCypher 13-0028 Normal Rabbit serum Invitrogen 01-610-1 Chemicals, Peptides, and Recombinant Proteins AzNTPs TriLink Technologies Cat# K-1005 Biotin-11-NTPs Perkin Elmer Cat# NEL54(2/3/4/5)001 Critical Commercial Assays NEBNext Ultra II DNA library kit NEB Cat# E7103S Deposited Data Raw and analyzed data This paper GEO: {"type":"entrez-geo","attrs":{"text":"GSE114467","term_id":"114467"}} GSE114467 Start-seq from Triptolide-treated S2 cells Krebs et al., 2017 GEO: {"type":"entrez-geo","attrs":{"text":"GSE77369","term_id":"77369"}} GSE77369 H3K4me1, H3K4me3 and H3K36me3 ChIP-seq from S2 cells Henriques et al., 2018 GEO: {"type":"entrez-geo","attrs":{"text":"GSE85191","term_id":"85191"}} GSE85191 Start-seq from Control and Rrp40-depleted S2 cells Henriques et al., 2013 GEO: {"type":"entrez-geo","attrs":{"text":"GSE49078","term_id":"49078"}} GSE49078 Chromatin RNA-seq from IntS11-depleted HeLa cells Lai et al., 2015 GEO: {"type":"entrez-geo","attrs":{"text":"GSE68401","term_id":"68401"}} GSE68401 H3K4me1, H3K4me3 and H3K36me3 ChIP-seq from HeLa cells Gerstein et al., 2012 GEO: {"type":"entrez-geo","attrs":{"text":"GSE29611","term_id":"29611"}} GSE29611 PRO-seq from DMSO-treated HeLa cells Nilson et al. 2017 GEO: {"type":"entrez-geo","attrs":{"text":"GSE100742","term_id":"100742"}} GSE100742 Chromatin RNA-seq from Xrn2 or CPS73-depleted HeLa cells Nojima et al., 2015 GEO: {"type":"entrez-geo","attrs":{"text":"GSE60358","term_id":"60358"}} GSE60358 Experimental Models: Cell Lines DL1 cells Dr. Sara Cherry, UPenn S2-DGRC clone 6 DGRC Stock number 6 DL1 FLAG-eGFP This paper DL1 FLAG-IntS11WT This paper DL1 FLAG-IntS11E203Q This paper Oligonucleotides Table S3 This paper Recombinant DNA pUB-3xFLAG vector Chen et al., 2012 pMT-puro David Sabatini lab Addgene #17923 pMT-FLAG-dIntS11-WT-puro This paper pMT-FLAG-dIntS11-E203Q-puro This paper pMT-FLAG-eGFP-puro This paper Software and Algorithms bowtie 1.2.2 Langmead et al., 2009 R v3.3.1 www.r-project.org Rstudio v1.0.136 www.rstudio.com featureCounts Liao et al., 2014 DESeq2 Love et al. , 2014 MISO Katz et al., 2010 Prism v8.1.2 GraphPad Partek Genomics Suite v6.15.0127 www.partek.com Open in a separate window KEY RESOURCES TABLE Integrator inhibits transcription elongation at ~15% of mRNA genes and enhancers Integrator targets promoter-proximally paused Pol II for termination The RNA endonuclease of Integrator subunit 11 is critical for gene repression Integrator-depletion increases productive elongation and Histone H3 K4 methylation

    Plasmid Preparation:

    Article Title: The Integrator complex attenuates promoter-proximal transcription at protein-coding genes
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IntS1 Ezzeddine et al., 2011 IntS9 Ezzeddine et al., 2011 IntS11 Ezzeddine et al., 2011 alpha-tubulin abcam ab15246 AB_301787 H3K4me3 EpiCypher 13-0028 Normal Rabbit serum Invitrogen 01-610-1 Chemicals, Peptides, and Recombinant Proteins AzNTPs TriLink Technologies Cat# K-1005 Biotin-11-NTPs Perkin Elmer Cat# NEL54(2/3/4/5)001 Critical Commercial Assays NEBNext Ultra II DNA library kit NEB Cat# E7103S Deposited Data Raw and analyzed data This paper GEO: {"type":"entrez-geo","attrs":{"text":"GSE114467","term_id":"114467"}} GSE114467 Start-seq from Triptolide-treated S2 cells Krebs et al., 2017 GEO: {"type":"entrez-geo","attrs":{"text":"GSE77369","term_id":"77369"}} GSE77369 H3K4me1, H3K4me3 and H3K36me3 ChIP-seq from S2 cells Henriques et al., 2018 GEO: {"type":"entrez-geo","attrs":{"text":"GSE85191","term_id":"85191"}} GSE85191 Start-seq from Control and Rrp40-depleted S2 cells Henriques et al., 2013 GEO: {"type":"entrez-geo","attrs":{"text":"GSE49078","term_id":"49078"}} GSE49078 Chromatin RNA-seq from IntS11-depleted HeLa cells Lai et al., 2015 GEO: {"type":"entrez-geo","attrs":{"text":"GSE68401","term_id":"68401"}} GSE68401 H3K4me1, H3K4me3 and H3K36me3 ChIP-seq from HeLa cells Gerstein et al., 2012 GEO: {"type":"entrez-geo","attrs":{"text":"GSE29611","term_id":"29611"}} GSE29611 PRO-seq from DMSO-treated HeLa cells Nilson et al. 2017 GEO: {"type":"entrez-geo","attrs":{"text":"GSE100742","term_id":"100742"}} GSE100742 Chromatin RNA-seq from Xrn2 or CPS73-depleted HeLa cells Nojima et al., 2015 GEO: {"type":"entrez-geo","attrs":{"text":"GSE60358","term_id":"60358"}} GSE60358 Experimental Models: Cell Lines DL1 cells Dr. Sara Cherry, UPenn S2-DGRC clone 6 DGRC Stock number 6 DL1 FLAG-eGFP This paper DL1 FLAG-IntS11WT This paper DL1 FLAG-IntS11E203Q This paper Oligonucleotides Table S3 This paper Recombinant DNA pUB-3xFLAG vector Chen et al., 2012 pMT-puro David Sabatini lab Addgene #17923 pMT-FLAG-dIntS11-WT-puro This paper pMT-FLAG-dIntS11-E203Q-puro This paper pMT-FLAG-eGFP-puro This paper Software and Algorithms bowtie 1.2.2 Langmead et al., 2009 R v3.3.1 www.r-project.org Rstudio v1.0.136 www.rstudio.com featureCounts Liao et al., 2014 DESeq2 Love et al. , 2014 MISO Katz et al., 2010 Prism v8.1.2 GraphPad Partek Genomics Suite v6.15.0127 www.partek.com Open in a separate window KEY RESOURCES TABLE Integrator inhibits transcription elongation at ~15% of mRNA genes and enhancers Integrator targets promoter-proximally paused Pol II for termination The RNA endonuclease of Integrator subunit 11 is critical for gene repression Integrator-depletion increases productive elongation and Histone H3 K4 methylation

    Software:

    Article Title: The Integrator complex attenuates promoter-proximal transcription at protein-coding genes
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IntS1 Ezzeddine et al., 2011 IntS9 Ezzeddine et al., 2011 IntS11 Ezzeddine et al., 2011 alpha-tubulin abcam ab15246 AB_301787 H3K4me3 EpiCypher 13-0028 Normal Rabbit serum Invitrogen 01-610-1 Chemicals, Peptides, and Recombinant Proteins AzNTPs TriLink Technologies Cat# K-1005 Biotin-11-NTPs Perkin Elmer Cat# NEL54(2/3/4/5)001 Critical Commercial Assays NEBNext Ultra II DNA library kit NEB Cat# E7103S Deposited Data Raw and analyzed data This paper GEO: {"type":"entrez-geo","attrs":{"text":"GSE114467","term_id":"114467"}} GSE114467 Start-seq from Triptolide-treated S2 cells Krebs et al., 2017 GEO: {"type":"entrez-geo","attrs":{"text":"GSE77369","term_id":"77369"}} GSE77369 H3K4me1, H3K4me3 and H3K36me3 ChIP-seq from S2 cells Henriques et al., 2018 GEO: {"type":"entrez-geo","attrs":{"text":"GSE85191","term_id":"85191"}} GSE85191 Start-seq from Control and Rrp40-depleted S2 cells Henriques et al., 2013 GEO: {"type":"entrez-geo","attrs":{"text":"GSE49078","term_id":"49078"}} GSE49078 Chromatin RNA-seq from IntS11-depleted HeLa cells Lai et al., 2015 GEO: {"type":"entrez-geo","attrs":{"text":"GSE68401","term_id":"68401"}} GSE68401 H3K4me1, H3K4me3 and H3K36me3 ChIP-seq from HeLa cells Gerstein et al., 2012 GEO: {"type":"entrez-geo","attrs":{"text":"GSE29611","term_id":"29611"}} GSE29611 PRO-seq from DMSO-treated HeLa cells Nilson et al. 2017 GEO: {"type":"entrez-geo","attrs":{"text":"GSE100742","term_id":"100742"}} GSE100742 Chromatin RNA-seq from Xrn2 or CPS73-depleted HeLa cells Nojima et al., 2015 GEO: {"type":"entrez-geo","attrs":{"text":"GSE60358","term_id":"60358"}} GSE60358 Experimental Models: Cell Lines DL1 cells Dr. Sara Cherry, UPenn S2-DGRC clone 6 DGRC Stock number 6 DL1 FLAG-eGFP This paper DL1 FLAG-IntS11WT This paper DL1 FLAG-IntS11E203Q This paper Oligonucleotides Table S3 This paper Recombinant DNA pUB-3xFLAG vector Chen et al., 2012 pMT-puro David Sabatini lab Addgene #17923 pMT-FLAG-dIntS11-WT-puro This paper pMT-FLAG-dIntS11-E203Q-puro This paper pMT-FLAG-eGFP-puro This paper Software and Algorithms bowtie 1.2.2 Langmead et al., 2009 R v3.3.1 www.r-project.org Rstudio v1.0.136 www.rstudio.com featureCounts Liao et al., 2014 DESeq2 Love et al. , 2014 MISO Katz et al., 2010 Prism v8.1.2 GraphPad Partek Genomics Suite v6.15.0127 www.partek.com Open in a separate window KEY RESOURCES TABLE Integrator inhibits transcription elongation at ~15% of mRNA genes and enhancers Integrator targets promoter-proximally paused Pol II for termination The RNA endonuclease of Integrator subunit 11 is critical for gene repression Integrator-depletion increases productive elongation and Histone H3 K4 methylation

    Methylation:

    Article Title: The Integrator complex attenuates promoter-proximal transcription at protein-coding genes
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IntS1 Ezzeddine et al., 2011 IntS9 Ezzeddine et al., 2011 IntS11 Ezzeddine et al., 2011 alpha-tubulin abcam ab15246 AB_301787 H3K4me3 EpiCypher 13-0028 Normal Rabbit serum Invitrogen 01-610-1 Chemicals, Peptides, and Recombinant Proteins AzNTPs TriLink Technologies Cat# K-1005 Biotin-11-NTPs Perkin Elmer Cat# NEL54(2/3/4/5)001 Critical Commercial Assays NEBNext Ultra II DNA library kit NEB Cat# E7103S Deposited Data Raw and analyzed data This paper GEO: {"type":"entrez-geo","attrs":{"text":"GSE114467","term_id":"114467"}} GSE114467 Start-seq from Triptolide-treated S2 cells Krebs et al., 2017 GEO: {"type":"entrez-geo","attrs":{"text":"GSE77369","term_id":"77369"}} GSE77369 H3K4me1, H3K4me3 and H3K36me3 ChIP-seq from S2 cells Henriques et al., 2018 GEO: {"type":"entrez-geo","attrs":{"text":"GSE85191","term_id":"85191"}} GSE85191 Start-seq from Control and Rrp40-depleted S2 cells Henriques et al., 2013 GEO: {"type":"entrez-geo","attrs":{"text":"GSE49078","term_id":"49078"}} GSE49078 Chromatin RNA-seq from IntS11-depleted HeLa cells Lai et al., 2015 GEO: {"type":"entrez-geo","attrs":{"text":"GSE68401","term_id":"68401"}} GSE68401 H3K4me1, H3K4me3 and H3K36me3 ChIP-seq from HeLa cells Gerstein et al., 2012 GEO: {"type":"entrez-geo","attrs":{"text":"GSE29611","term_id":"29611"}} GSE29611 PRO-seq from DMSO-treated HeLa cells Nilson et al. 2017 GEO: {"type":"entrez-geo","attrs":{"text":"GSE100742","term_id":"100742"}} GSE100742 Chromatin RNA-seq from Xrn2 or CPS73-depleted HeLa cells Nojima et al., 2015 GEO: {"type":"entrez-geo","attrs":{"text":"GSE60358","term_id":"60358"}} GSE60358 Experimental Models: Cell Lines DL1 cells Dr. Sara Cherry, UPenn S2-DGRC clone 6 DGRC Stock number 6 DL1 FLAG-eGFP This paper DL1 FLAG-IntS11WT This paper DL1 FLAG-IntS11E203Q This paper Oligonucleotides Table S3 This paper Recombinant DNA pUB-3xFLAG vector Chen et al., 2012 pMT-puro David Sabatini lab Addgene #17923 pMT-FLAG-dIntS11-WT-puro This paper pMT-FLAG-dIntS11-E203Q-puro This paper pMT-FLAG-eGFP-puro This paper Software and Algorithms bowtie 1.2.2 Langmead et al., 2009 R v3.3.1 www.r-project.org Rstudio v1.0.136 www.rstudio.com featureCounts Liao et al., 2014 DESeq2 Love et al. , 2014 MISO Katz et al., 2010 Prism v8.1.2 GraphPad Partek Genomics Suite v6.15.0127 www.partek.com Open in a separate window KEY RESOURCES TABLE Integrator inhibits transcription elongation at ~15% of mRNA genes and enhancers Integrator targets promoter-proximally paused Pol II for termination The RNA endonuclease of Integrator subunit 11 is critical for gene repression Integrator-depletion increases productive elongation and Histone H3 K4 methylation



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    pmt flag dints11 wt puro  (Addgene inc)
    93
    Addgene inc pmt flag dints11 wt puro
    KEY RESOURCES TABLE
    Pmt Flag Dints11 Wt Puro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmt+flag+dints11+wt+puro/pMT-puro+(Plasmid+%2317923)/pmc06952639-1085-216-214
    Average 93 stars, based on 1 article reviews
    pmt flag dints11 wt puro - by Bioz Stars, 2026-10
    93/100 stars
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    KEY RESOURCES TABLE

    Journal: Molecular cell

    Article Title: The Integrator complex attenuates promoter-proximal transcription at protein-coding genes

    doi: 10.1016/j.molcel.2019.10.034

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IntS1 Ezzeddine et al., 2011 IntS9 Ezzeddine et al., 2011 IntS11 Ezzeddine et al., 2011 alpha-tubulin abcam ab15246 AB_301787 H3K4me3 EpiCypher 13-0028 Normal Rabbit serum Invitrogen 01-610-1 Chemicals, Peptides, and Recombinant Proteins AzNTPs TriLink Technologies Cat# K-1005 Biotin-11-NTPs Perkin Elmer Cat# NEL54(2/3/4/5)001 Critical Commercial Assays NEBNext Ultra II DNA library kit NEB Cat# E7103S Deposited Data Raw and analyzed data This paper GEO: {"type":"entrez-geo","attrs":{"text":"GSE114467","term_id":"114467"}} GSE114467 Start-seq from Triptolide-treated S2 cells Krebs et al., 2017 GEO: {"type":"entrez-geo","attrs":{"text":"GSE77369","term_id":"77369"}} GSE77369 H3K4me1, H3K4me3 and H3K36me3 ChIP-seq from S2 cells Henriques et al., 2018 GEO: {"type":"entrez-geo","attrs":{"text":"GSE85191","term_id":"85191"}} GSE85191 Start-seq from Control and Rrp40-depleted S2 cells Henriques et al., 2013 GEO: {"type":"entrez-geo","attrs":{"text":"GSE49078","term_id":"49078"}} GSE49078 Chromatin RNA-seq from IntS11-depleted HeLa cells Lai et al., 2015 GEO: {"type":"entrez-geo","attrs":{"text":"GSE68401","term_id":"68401"}} GSE68401 H3K4me1, H3K4me3 and H3K36me3 ChIP-seq from HeLa cells Gerstein et al., 2012 GEO: {"type":"entrez-geo","attrs":{"text":"GSE29611","term_id":"29611"}} GSE29611 PRO-seq from DMSO-treated HeLa cells Nilson et al. 2017 GEO: {"type":"entrez-geo","attrs":{"text":"GSE100742","term_id":"100742"}} GSE100742 Chromatin RNA-seq from Xrn2 or CPS73-depleted HeLa cells Nojima et al., 2015 GEO: {"type":"entrez-geo","attrs":{"text":"GSE60358","term_id":"60358"}} GSE60358 Experimental Models: Cell Lines DL1 cells Dr. Sara Cherry, UPenn S2-DGRC clone 6 DGRC Stock number 6 DL1 FLAG-eGFP This paper DL1 FLAG-IntS11WT This paper DL1 FLAG-IntS11E203Q This paper Oligonucleotides Table S3 This paper Recombinant DNA pUB-3xFLAG vector Chen et al., 2012 pMT-puro David Sabatini lab Addgene #17923 pMT-FLAG-dIntS11-WT-puro This paper pMT-FLAG-dIntS11-E203Q-puro This paper pMT-FLAG-eGFP-puro This paper Software and Algorithms bowtie 1.2.2 Langmead et al., 2009 R v3.3.1 www.r-project.org Rstudio v1.0.136 www.rstudio.com featureCounts Liao et al., 2014 DESeq2 Love et al. , 2014 MISO Katz et al., 2010 Prism v8.1.2 GraphPad Partek Genomics Suite v6.15.0127 www.partek.com Open in a separate window KEY RESOURCES TABLE Integrator inhibits transcription elongation at ~15% of mRNA genes and enhancers Integrator targets promoter-proximally paused Pol II for termination The RNA endonuclease of Integrator subunit 11 is critical for gene repression Integrator-depletion increases productive elongation and Histone H3 K4 methylation

    Techniques: Recombinant, Plasmid Preparation, Software